Author/Editor     Petrovec, M; Sumner, JW; Nicholson, WL; Childs, JE; Strle, F; Barlič, J; Lotrič-Furlan, S; Avšič-Županc, T
Title     Identity of ehrlichial DNA sequences derived from Ixodes ricinus ticks with those obtained from patients with human granulocytic ehrlichiosis in Slovenia
Type     članek
Source     J Clin Microbiol
Vol. and No.     Letnik 37, št. 1
Publication year     1999
Volume     str. 209-10
Language     eng
Abstract     Adult ixodes ricinus (Acari: Ixodidae) ticks collected near Ljubljana, Slovenia, were tested for the agent of human granulocytic ehrlichiosis (HGE) by using PCR assays based on the 16S rRNA gene. Three (3.2%) of 93 ticks were found to contain granulocytic ehrlichiae. Nucleotide sequences of portions of the bacterial groESL heat shock operon amplified from these ticks were identical or nearly (99.8%) identical to those previously determined ror human patients with HGE from Slovenia, providing additional evidence that the ticks were infected with the HGE agent. This study identified I. ricinus a sthe likely vector for these ehrlichial pathogens of humans in this part of Europe.
Descriptors     EHRLICHIOSIS
IXODES
RNA, RIBOSOMAL, 16S
POLYMERASE CHAIN REACTION
BASE SEQUENCE
SEQUENCE ANALYSIS, DNA
ARACHNID VECTORS