Author/Editor		Cimerman, Nina; Trstenjak-Prebanda, Mojca; Turk, Boris; Popovič, Tatjana; Dolenc, Iztok; Turk, Vito
Title		Interaction of cystatin C variants with papain and human cathepsins B,H and L
Type		članek
Source		J Enzym Inhib
Vol. and No.		Letnik 14
Publication year		1999
Volume		str. 167-74
Language		eng
Abstract		Recombinant human cystatin C and two of its mutants were expressed in Escherichia coli. The recombinant inhibitor was found to identical to authentic cystatin C as judged by isoelectric focusing (pI 9.2) and kinetics of inhibition of papain and human cathepsins B, H and L. N-terminal truncation of 8 residues resulted in a decrease of isoelectric point (pI 7.8), but the inhibitory properties were similar to those of recombinant cystatin C, suggesting that Leu9 is a critical residue for the inhibition. The mutation of Trp106 to Ser, however, resulted in a decreased affinity of the inhibitor for the enzymes tested, with the largest effect on cathepsin B inhibition (100-fold in increase in Ki).
Descriptors		CYSTATINS CATHEPSIN B ENZYME INHIBITORS PAPAIN BACTERIAL PROTEINS ESCHERICHIA COLI PROTEINS