| Author/Editor | Erdani-Kreft, Mateja | |
| Title | Vloga laminae propriae pri razvoju in diferenciaciji urotelijskih celic sečnega mehurja miši in vitro | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 1999 | |
| Volume | str. 87 | |
| Language | slo | |
| Abstract | The role of lamina propria and epidermal growth factor (EGF) in development and differentiation of urothelial cells in the urinary bladder in vivo and in vitro is poorly understood. The purpose of this study was to determine the effect of lamina propria and EGF on growth, differentiation and epithelial morphology of urothelial cells in cultures of mouse urinary bladder. Urothelial tissue cultures were maintained on a Cyclopore membrane in serum-free medium for 10 days, and thereafter analysed by light and electron microscopy using morphological, immunohistochemical and immunocytochemical methods. We found out that exogenously added EGF has no affect either on cell proliferation or differentiation. Therefore, we distinguished only between cultures which were growing in presence or absence of lamina propria. Our results show that urothelial cells, which grow with lamina propria (culture type I), have better growth and are more differentiated than urothelial cells, which grow with lamina propria only for first four days (culture type II). Urothelial cells, which grow all 10 days without lamina propria, are nondifferentiated. Urothelial cells in cultures type I and II show terminal differentiation from the Cyclopore membrane toward the surface. On the surface of the new multilayer epithelium are the most differentiated cells with apical membrane shaped in microvilli, ropy microridges, rounded microridges or scalloped microridges. Immunohistochemical and immunocytochemical analysis of uroplakins ,show that cells with asymmetricaly thickened membrane shaped in scalloped microridges are terminally differentiated. This is the first description of the syntesis of uroplakins and their assembly in the asymmetricaly thickened membrane in urothelial cells in vitro. (Abstract truncated at 2000 characters) | |
| Descriptors | BLADDER UROTHELIUM EPIDERMAL GROWTH FACTOR-UROGASTRONE MICE ADULT CELL DIFFERENTIATION CELL DIVISION TISSUE CULTURE IMMUNOHISTOCHEMISTRY DESMOSOMES INTERMEDIATE FILAMENT PROTEINS MICROSCOPY, ELECTRON |