Author/Editor		Plemenitaš, Ana; Lu, Xiaobin; Geyer, Matthias; Veranič, Peter; Simon, Marie-Noelle; Peterlin, B Matija
Title		Activation of the Ste20 by Nef from human immunodeficiency virus induces cytoskeleton rearrangements and downstream effector functions in Saccharomyces cerevisiae
Type		članek
Source		Virology
Vol. and No.		Letnik 258
Publication year		1999
Volume		str. 271-81
Language		eng
Abstract		The negative factor (Nef) from human and simian immunodeficiency viruses is important for the pathogenesis of aciquired immune deficiency syndrome. Among other target, it activates the Nef-associated kinase, wich is related to the p21-activated kinase. In this study, we demonstrate that Nef activates Ste20, the homolog of p21-activated kinase in Saccharomyces cerevisiae. Nef binds to the adaptor proteins Bem 1 and Ste20 via its proline-rich (PXXP) and diarginine (RR) motifs, respectively. These interactions induce the mitogen-activated protein kinase and increase the rates of budding, sizes of cells, and patterns of mating projections. These effects of Nef depend on the small GTPase Cdc42 and guanine nucleotide exchange factoe Cdc24. Thus, studies in S. cerevisiae identified specific interactions between Nef and cellular proteins and their associated signaling casade.
Descriptors		HIV SACCHAROMYCES CEREVISIAE PROTEIN-SERINE-THREONINE KINASES CYTOSKELETON GENE PRODUCTS, NEF AMINO ACID SEQUENCE CELL CYCLE PROTEINS ENZYME ACTIVATION FUNGAL PROTEINS G-PROTEINS MOLECULAR SEQUENCE DATA PHEROMONES