Author/Editor		Bantan, Tjaša; Milačič, Radmila; Pihlar, Boris
Title		Kvantitativna določitev Al-citrata v krvnem serumu z uporabo FPLC in ETAAS
Type		članek
Source		In: Glavič P, Brodnjak-Vončina D, editors. Zbornik referatov s posvetovanja Slovenski kemijski dnevi 1998; 1998 sep 17-18; Maribor. Maribor: FKTT,
Publication year		1998
Volume		str. 60-5
Language		slo
Abstract		An anionexchange fast protein liquid chromatographic - electrothermal atomic absorption spectrometric procedure (FPLC - ETAAS) was developed for determination of trace amounts of negatively charged A1-citrate. Aqueous - 4 mol dm-3 NH4NO3 linear gradient elution at a flow rate of 1 cm3 min-1 was applied for 10 min to separate AI-citrate on a FPLC Mono Q HR 5/5 column. The separated aluminium species were determined "off line" by ETAAS in 0.5 cm3 fractions. Using the procedure developed reproducible (RSD +- 2.0%) and quantitative determination of negatively charged Al-citrate at a retention time of 4.5 min was obtained. The LOD was found to be 2.0 ng cm-3 of A1-citrate. The technique was successfully applied for the determination of AI-citrate in human serum. Spiked samples (50-150 ng Al3+cm-3) were microultrafiltered through a membrane filter (cut-off 30000 Da) to separate aluminium bound to transferrin from low molecular weight aluminium complexes. It was found that 15 +- 0,5 % of aluminium in spiked samples from healthy volunteers passed through the membrane. By applying FPLC separation it was proved that all the aluminium in the filtrate corresponded to A1-citrate.
Descriptors		ALUMINUM CITRATES SPECTROPHOTOMETRY, ATOMIC ABSORPTION