Author/Editor		Mašera, Andrej; Staniša, Olga; Ovčak, Zdenka; Coer, Andrej
Title		Effect pf pH on Ki-67 antigen retrieval in renal cell carcinoma
Type		članek
Source		Acta Med Croatica
Vol. and No.		Letnik 53, št. 1
Publication year		1999
Volume		str. 15-7
Language		eng
Abstract		The efficacy of Ki--67 antigen retrieval (AR) was assessed in 20 formalin fixed-paraffin embedded specimens ot renal cell carcinoma using three solutions with different pH values. The AR was performed by means of microwave oven heating up to 97 degree C for 15 minutes. Better results were obtained using solutions with pH values 8.0 and 9.9 compared with AR tn a solutton of pH 6.0, which is routinely used in daily pathological practice. Individual testing of various AR conditlons is recommended to obtain the best immunohistochemical results.
Descriptors		CARCINOMA, RENAL CELL KI-67 ANTIGEN KIDNEY NEOPLASMS HYDROGEN-ION CONCENTRATION IMMUNOHISTOCHEMISTRY