Author/Editor		Žnidaršič, Polona; Komel, Radovan; Pavko, Aleksander
Title		Studies of a pelleted growth from of Rhizopus nigricans as a biocatalyst for progesterone 11alpha-hydroxylation
Type		članek
Source		J Biotechnol
Vol. and No.		Letnik 60
Publication year		1998
Volume		str. 207-16
Language		eng
Abstract		The noncoagulative type of pellet formation can be induced in submergcd cultivation of the filamentous fungus Rhizopus nigricans. The size and constitution of the hyphal agglomerates obtained varied with changes in inoculum size and agitation speed for given media composition and cultivation conditions. The physiological state of mycelium, used for a further process of biotransformation, was estimated by following thc growth kinetics, pH value and substrate utilization during submerged cultivation. Namely, differenoes in pcllet morphology and physiology affect the ability of R. nigricans to hydroxylate progesterone at the lla position. A repeated batch procedure revealed the best maintenance of biotransformation capacity for pellets, obtaiaed from the growth phase of cultivation at high agitation speed and with low inoculum size.
Descriptors		RHIZOPUS PROGESTERONE HYDROXYPROGESTERONES HYDROXYLATION BIOTRANSFORMATION CULTURE MEDIA HYDROGEN-ION CONCENTRATION BIOMASS