Author/Editor		Vodopivec, Boris
Title		Spremembe v stromi pri maligni preobrazbi polupoidnih adenomov širokega črevesa in danke
Translated title		The stromal changes associated with the malignant change of colorectal polypoid adenomas
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1999
Volume		str. 77
Language		slo
Abstract		Backround. Tumor stroma is a specialised tissue important for the tumor growth and nutrition. We have analysed stromal changes in early polypoid colorectal adenocarcinomas (PCRC). One of the main questions was the difference in the stroma composition between early PCRC and benign colorectal polyps. Methods and materials. A retrospective analysis was performed on biopsies of early colorectal polypoid adenocarcinoma (Dukes A, TI=72%, T2=28%). Adenomas with early malignant change (n=25), and three control groups of polyps with stromal changes, adenomas (n=12), juvenile polyps (n=11) and polyps connected with rectal prolapse (n+11) were included in the study. Biopsy tissue specimens, fixed in neutral formalin and embedded in paraffin, were stained with haematoxilin and eosin, and Kreyberg methods. Stromal changes were analysed by immunohistochemical methods using the following antigens: alpha-smooth muscle actin (alpha-SMA), von Willebrand factor (vWF), desmin, fibrinoctin, collagen III, collagen IV, laminin, Ki-67, CD68 and by the reaction with lectin Ulex Europaeus agglutinin-1 (UEA-1) for alpha-L-fucose. For statistical analysis, a nonparametric Wilcoxon rank sum test and Mann-Whitney test-U were used. The criterion for the statistical significance was P<0,05. Results. We have found the following stromal changes in the early PCRC, that were significantly different from the control groups of polyps: The number of myofibroblasts and pericytes was increased. They were arranged in multiple layers around tumor glands. In the myofibroblast cytoplasm, alpha-SMA but no desmin, was found. The orientation of myofibroplasts, pericytes and smooth muscle cells was irregular. Small vessels were increased in number and their morphology as well as architecture were altered in a characteristic way. The capillaries were arranged around carcinomatous glands in multiple layers and showed characteristic budding in all directions. (Abstract truncated at 2000 characteristics.)
Descriptors		COLORECTAL NEOPLASMS ADENOMATOUS POLYPOSIS COLI INTESTINAL POLYPS BIOPSY IMMUNOHISTOCHEMISTRY PROSPECTIVE STUDIES VON WILLEBRAND FACTOR DESMIN TENASCIN COLLAGEN KI-67 ANTIGEN LAMININ LECTINS