| Author/Editor | Kuret, Jošt | |
| Title | Morfologija in ultrastruktura bakterije Borrelia burgdorferi s.l. in njene spremembe po delovanju nekaterih antibiotikov | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 1999 | |
| Volume | str. 117 | |
| Language | slo | |
| Abstract | In this work we are presenting the results of our study of morphology and ultrastructure of Borrelia burgdorferi sensu lato. According to available data, borrelia is morphologically very variable. Morphological alterations are caused by chemical in bacterial environment. Bubble-like structures of the other sheath, called blebs, small bubbles, budding from outer sheath and altered morphology of protoplasmic cilinder are most frequently reported alterations. Our intention was to morphology and ultrastructure of chemically prepared bacteria with morphology and ultrastructure of high pressure frozen and freeze substituted B. burgdorferi s.l. by transmission electron microscope. We wanted to determine if some of the morphological changes, descibed above are result of chemical methods for electron microscopy. The results of three experiments served as the basis for studying the influence of antibiotics on morphology and ultrastructure of B. burgdorferi s.l. Negative staining method provided basic data on morphology of borrelia (length, width, helical shape of protoplasmic cilinder, position of ultrastructural elements). To compare influence of different electron microscopical methods on morphology and ultrastructure of bacterium, we took bacteria from growth medium and placed them in capillary cellulose tubes with inner diameter of 200 micro m. Tubes were subsequently sealed and divided in two groups. One group was processed by chemical methods and the other by freezing techniques. For chemical methods we chose aldehyde fixation, followed by dehydration in series of ethanol. Finally, we embedded bacteria in epoxy resin for studies of ultrastructure or in LR White for immunocytochemical studies. ( Abstracts truncated at 2000 characters.) | |
| Descriptors | BORRELIA BURGDORFERI MICROSCOPY, ELECTRON ANTIBIOTICS CRYOPRESERVATION FREEZE SUBSTITUTION AZITHROMYCIN CEFTRIAXONE DOXYCYCLINE IMMUNOHISTOCHEMISTRY ANTIBODIES, MONOCLONAL ANTIGENS, BACTERIAL EPOXY RESINS |