Author/Editor | Geršak, Ksenija; Seppaelae, Markku; Us-Krašovec, Marija | |
Title | The same luteinized granulosa-luteal cells from normal and superovulated human chorionic gonadotropin (hCG)-stimulated cycles contain hCG and insulin-like growth factor binding protein-1 | |
Type | članek | |
Source | Fertil Steril | |
Vol. and No. | Letnik 71, št. 5 | |
Publication year | 1999 | |
Volume | str. 902-6 | |
Language | eng | |
Abstract | Objective: To determine whether hCG and IGFBP-1 appear in the same or different cells and in what sequence. Design: Retrospective analysis of laboratory data. Setting: University medical center. Patient(s): Twenty-five women undergoing IVF-ET with natural cycles and 25 women having stimulated IVF-ET. Intervention(s): Cells were obtained from dominant follicles in women with natural cycles and from the follicles from hMG- and hCG-stimulated cycles. Main Outcome Measure(s): Detection and localization of hCG and IGFBP-1 in granulosa-luteal cells using double immunocytochemical staining. Measurement of hCG and IGFBP-1 in follicular fluid and serum. Result(s): Three types of hCG staining were found: on the cell surface, on the cell surface and in the cytoplasm, and in the cytoplasni alone. IGFBP-1 stained diffusely in the cytoplasm and was fund only in those cells that were luteinized and contained hCG. IGFBP-1 and hCG were colocalized in the same cells. There was a positive correlation between follicular fluid hCG and IGFBP-1 levels, but only in natural IVF-ET cycles. Conclusion(s): HCG-driven Iuteinization is required for IGFBP-1 synthesis to take place in granulosa cells. | |
Descriptors | GONADOTROPINS, CHORIONIC INSULIN-LIKE GROWTH-FACTOR BINDING PROTEIN 1 GRANULOSA CELLS LUTEIN CELLS OVULATION INDUCTION ADULT IMMUNOHISTOCHEMISTRY RETROSPECTIVE STUDIES FERTILIZATION IN VITRO EMBRYO TRANSFER |