Author/Editor		Čemažar, M; Miklavčič, D; Ščančar, J; Dolžan, V; Golouh, R; Serša, G
Title		Increased platinum accumulation in SA-1 tumour cells after in vivo electrochemotherapy with cisplatin
Type		članek
Source		Br J Cancer
Vol. and No.		Letnik 79, št. 9-10
Publication year		1999
Volume		str. 1386-91
Language		eng
Abstract		Electrochemotherapy is an anti-tumour treatment that utilizes locally delivered electric pulses to increase cytotoxicity of chemotherapeutic drugs. The aim of our study was to determine whether anti-tumour effectiveness of electrochemotherapy with cisplatin is a consequence of increased plasma membrane permeability caused by electroporation that enables cisplatin binding to DNA. For this purpose, anti-tumour effectiveness of electrochemotherapy was evaluated on SA-1 tumours treated with electric pulses 3 min affer intravenous injection of cisplatin (4 mg kg-1). Anti-tumour effectiveness was correlated with platinum accumulation in tumours and the amount of platinum bound to DNA, as determined by atomic absorption spectrometry. In tumours treated with electrochemotherapy, cell kill was increased by a factor of 20 compared with treatment with cisplatin only, as determined from tumour growth curves. The amount of platinum bound to DNA and platinum content in the tumours treated by electrochemotherapy was approximately two times higher than in cisplatin-treated tumours. Based on our results, we conclude that in vivo application of electric pulses potentiates anti-tumour effectiveness of cisplatin by electroporation that consequently results in cisplatin increased delivery into the cells. In addition, besides electroporation, immune system and tumour blood flow changes could be involved in the observed anti-tumour effectiveness of electrochemotherapy.
Descriptors		SARCOMA, EXPERIMENTAL ELECTRIC STIMULATION THERAPY ELECTROPORATION CISPLATIN PLATINUM MICE