Author/Editor | Blank, Miri; Krause, Ilan; Goldkorn, Tzipora; Praprotnik, Sonja; Livneh, Avi; Langevitz, Pnina; Kaganovsky, Ella; Morrgenstern, Sara; Cohen, Shlomo; Barak, Vivian | |
Title | Monoclonal anti-endothelial cell antibodies from a patient with takayasu arteritis activate endothelial cells from large vessels | |
Type | članek | |
Source | Arthritis Rheum | |
Vol. and No. | Letnik 42, št. 7 | |
Publication year | 1999 | |
Volume | str. 1421-31 | |
Language | eng | |
Abstract | Objective. To create monoclonal anti-endothelial cell antibodies (mAECA) from a patient with Takayasu arteritis to evaluate their ability to activate human umbilical vein endothelial cells (HUVEC), and to characterize the mechanism of EC activation. Methods. A panel of mAECA was generated from peripheral blood lymphocytes of a patient with Takayasu arteritis, using Epstein-Barr virus transformation. Activity against macrovascular EC (HUVEC) and microvascular EC (human bone marrow EC immortalized by SV4O) antigens was detected by enzyme-linked immunosorbent assay. lnhibition studies were used to select the monoclonal antibodies (mAECA) which share the same EC epitope binding specificity as the total IgG-AECA from the Takayasu arteritis patient. The binding of the mAECA to human aortic EC was studied by immunohistochemistry. The secretion levels of interleukin-6 (IL-6) and von Willebrand factor (vWF) were determined, to serve as markers for EC activation. The activated EC were examined for the adherence of a monocytic cell line (U937), as well as for expression of vascular cell adhesion molecule1, intercellular adhesion molecule 1, and E-selectin. In addition, nuclear extracts of the mAECA-treated EC were analyzed for the induction of translocation of nuclear factor kB (NF-kB), using a specific NF-kB oligoprobe in an electophoretic mobility shift assay. (Abstract truncated at 2000 characters.) | |
Descriptors | TAKAYASU'S ARTERITIS ANTIBODIES, MONOCLONAL ENZYME-LINKED IMMUNOSORBENT ASSAY IMMUNOHISTOCHEMISTRY IGG ENDOTHELIUM, VASCULAR AORTA |