Author/Editor		Bjugn, Roger; Hagen, Maria; Sadeghi, Siamak; Nyengaard, Jens R
Title		A "recipe" for estimating neuron number in the mammalian spinal cord using modern stereological techniques
Type		članek
Source		Acta Stereol
Vol. and No.		Letnik 18, št. 2
Publication year		1999
Volume		str. 131-48
Language		eng
Abstract		In this review article a brief introduction to the organisation and function of the mammalian spinal cord is given. This short account is followed by a detailed practical approach for estimating the number of neurons, glial and endothelial cells in the grey matter of the spinal cord. All steps, from vascular perfusion fixation, removal of the spinal cord from the spinal column, identification and isolation of individual spinal cord segments, embedding, sectioning, staining, and the final stereological methods and statistical analysis are covered. Focus will be on the practical procedures, and the biological data obtained by these methpds will only be discussed with respect to some methodological problems that have been encountered. The possibility of combing specific cell labelling techniques, such as tract tracing and immunocytochemistry, with stereological methods is also discussed, as these methods may solve some of the uncertainties and problems facing neuroscientists who want estimates of neuron number.
Descriptors		SPINAL CORD NEURONS NEUROGLIA ADULT CELL COUNT MICE RATS RABBITS CATS IMMUNOHISTOCHEMISTRY