Author/Editor		Hagerstrand, Henry; Danieluk, Malgorzata; Bobrowska-Haegerstrand, Malgorzata; Pector, Veronique; Ruysschaert, Jean-Marie; Kralj-Iglič, Veronika; Iglič, Aleš
Title		Liposomes composed of a double-chain cationic amphiphile (vectamidine) induce their own encapsulation into human erythrocytes
Type		članek
Source		Biochim Biophys Acta
Vol. and No.		Letnik 1421
Publication year		1999
Volume		str. 125-30
Language		eng
Abstract		Vectamidine is a liposome-forming double-chain cationic amphiphile. The present work was aimed to microscopically study the interactions of Vectamidine liposomes with the human erythrocyte plasma membrane. Vectamidine rapidly induced stomatocytic shapes. Attachment of Vectamidine liposomes to the erythrocyte induced a strong local investigation of the membrane. This frequently resulted in a complete encapsulation of the liposome. Liposomes composed of phosphatidylcholine (neutral) or phosphatidylserine/phosphatidylcholine (anionic) did not perturb the erythsocyte shape. Our results indicate the besides an attraction of Vectamidine liposomes to the plasma membrane, there is a preference of Vectamidine for the inner bilayer laeaflet. We suggest that cationic amphiphiles may transfer from membrane-attached liposomes to the plasma membrane and then translocate to the inner bilayer leaflet where they induce a strong local inward bending of the plasma membrane resulting in an encapsulation of the liposome.
Descriptors		ERYTHROCYTES LIPOSOMES ERYTHROCYTE MEMBRANE CATIONS CELL SIZE PHOSPHATIDYLCHOLINES PHOSPHATIDYLSERINES