Author/Editor		Ihan-Hren, N; Gubina, M; Ihan, A
Title		Cytotoxic T lymphocytes versus streptococcal colonization in periapical granulomas
Type		članek
Source		J Endod
Vol. and No.		Letnik 25, št. 4
Publication year		1999
Volume		str. 239-42
Language		eng
Abstract		Seventeen dental periapical lesions were investigated to study bacterial colonization. Periapical lesions, obtained after apicotomy, were also enzymatically desegregated to quantitatively analyze lymphocyte subpopulations by flow cytometry. Fourteen samples yielded a positive bacterial growth when homogenized and cultured. We iso- lated enough lymphocytes to make flow cytometric analysis from 12 samples. A significant increase in interleukin-2 receptor and ICAM-1 molecule expression on T cells was found, compared with peripheral blood lymphocytes. Furthermore, a de- creased expression of interleukin-2 receptors and HLA DR molecules on CD8+ T cells was found in granulomas predominantly colonized by Streptococcus spp., compared with lesions predominantly colonized by strict anaerobes.
Descriptors		PERIAPICAL GRANULOMA CD8-POSITIVE T-LYMPHOCYTES STREPTOCOCCUS BACTERIA, AEROBIC CELL SEPARATION FLOW CYTOMETRY HLA-DR ANTIGENS INTERCELLULAR ADHESION MOLECULE-1 LYMPHOCYTE COUNT LYMPHOCYTE TRANSFORMATION RECEPTORS, INTERLEUKIN-2 TISSUE CULTURE