| Author/Editor | Zajc-Kreft, Katarina | |
| Title | Razgradnja acetilholinesterazne mRNA v normalni in denervirani skeletni mišici | |
| Translated title | Degradation of acetylcholinesterase mRNA in the normal and denervated skeletal muscle | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
| Publication year | 1999 | |
| Volume | str. 71 | |
| Language | slo | |
| Abstract | In broad terms, our research deals with the mechanisms regulating expression of mammalian neuromuscular junction components. We have focused our investigations on the mechanisms regulating acetylcholinesterase (AChE) expression in adult rat skeletal muscle. Our aim was to elucidate the mechanism of postdenervational decrease of AChE mRNA in the muscle, which is in a seeming contradiction with several reports demonstrating that AChE mRNA is downregulated by electromechanical activity. On the basis of these reports one would expect increased and not decreased AChE mRNA level in the inactive, denervated muscle. It could be hypothesized that denervation decreases AChE mRNA through some yet unidentified mechanisms, which seem to be strong enough to override upregulating effect of electromechanical inactivity. One possible mechanism that could explain decreased level of AChE mRNA in the denervated muscle is increased rate of its degradation. In order to test this possibility we first developed and tested a suitable method for the determination of AChE mRNA. A nonradioactive RNA blot hybridization, which was expected to be more convenient for our purposes than its radioactive version was selected. In the second part of our thesis we tested the hypothesis that in the denervated rat muscle the degradation of AChE mRNA is faster than in normal muscle. To elucidate the mechanism of AChE mRNA decrease we first measured the level of AChE mRNA at the selected time intervals after deneivation. Then we developed an in vitro system, in which deproteinated AChE mRNA was degradated with postmitochondrial fraction and its two subfractions, all isolated from the adult rat sternomastoideus (SM) muscle. (Abstract truncated at 2000 characters.) | |
| Descriptors | ACETYLCHOLINESTERASE MUSCLE DENERVATION RNA, MESSENGER POLYRIBOSOMES TRANSCRIPTION, GENETIC RATS HYBRIDIZATION DENSITOMETRY HISTOCYTOCHEMISTRY RNA PROBES DIGOXIGENIN DNA PROBES |