Author/Editor		Bračič, Katarina; Gregorič, Alojz; Vizjak, Alenka; Nagode, Breda
Title		Antinevtrofilna citoplazemska protitelesa (ANCA) pri otrocih s purpuro Schoenlein-Henoch in prizadetostjo ledvic
Translated title		Antineutrophil cytoplasmic antibodies (ANCA) in children with past history of Schoenlein-Henoch purpura and renal involvement
Type		članek
Source		In: Vizjak A, Rozman B, Ferluga D, editors. Autoimmune systemic diseases. Proceedings of the 30th memorial meeting for professor Janez Plečnik: under the auspices of the European society of pathology; 1999 Dec 2-3; Ljubljana. Ljubljana: Institute of pathology,
Publication year		1999
Volume		str. 33-8
Language		slo
Abstract		We investigated the frequency, isotype and antigen specificity of ANCA in sera of 20 children with haematuria and/or proteinuria after and acute episode of PSH and in 15 healthy age-matched controls. The pateint's sera were tested for perinuclear (P-ANCA), cytoplasmic (C-ANCA), and atypical ANCA by indirect immunofluorescence (IIF). By enzyme linked-immunosorbent assay (ELISA) the specificity for myeloŠeroxidase, proteinase 3, elstase, lactoferrin, capesin G, lyzosome was examined. Twenty children aged 4-15.2 years, mean 8.8 years 12 F, 8 M, with past history of PSH and persistence of urinary abnormalities on follow-up (6-40 months, mean 18 M). 17/20 had isolated microhaematuria, 1/20 had recurrent macrohaematuria, 1/20 had recurrent macrohematuria and proteinuria, and 1/20 had isolated proteinuria. IIF revealed weakly positive ANCA in 9/20 patient sera and none in 15 healthy controls. 1/9 sera showed P-ANCA, and 8/9 sera showed atypical ANCA (with homogenous cytoplasmic pattern), 2 of which were IgG and IgA positive, and 6 sera IgA positive. ANtigen specificity was tested by ELISA for mieloperoxidase, proteinase 3, lactoferrin. elastase, catepsin G, lysozyme and none yielded a positive result. We concluded> The absence of C-ANCA in all patients sera assist us in differentiating between PSH and Wegener. The lack of correlation of P-ANCA and atypical ANCA with any ANCA specifity (MPO, PR3, lactoferrin, elastase, catepsin G, lysozyme) suggests that circulating IgA-ANCA may be directed against a yet unknown antigen.
Descriptors		ANTIBODIES, ANTINEUTROPHIL CYTOPLASMIC PURPURA, SCHOENLEIN-HENOCH KIDNEY DISEASES FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT SLOVENIA CHILD IGA HEMATURIA VASCULITIS