Author/Editor     Rotter, Aleksander; Keše, Darja; Pezdirc, Magda; Batista, Urška; Jurčić, Vesna
Title     E-selectin and VCAM-1 expression of human umbilical vein endothelial cell (HUVEC) culture after the stimulation with Borrelia burgdorferi
Type     članek
Source     In: Vizjak A, Rozman B, Ferluga D, editors. Autoimmune systemic diseases. Proceedings of the 30th memorial meeting for professor Janez Plečnik; 1999 Dec 2-3; Ljubljana. Ljubljana: Faculty of medicine, Institute of pathology,
Publication year     1999
Volume     str. 45-9
Language     eng
Abstract     Endothelial cells (ECs) play a crucial role in triggering inflammatory responses, and they are the most important factor in controlling leukocyte traffic (1). Either directly by injury or by several inflammatory cytokines, activated ECs induce the expression of endothelial - leukocyte adhesion molecules (AMs) and the secretion of chemotactic factors for neutrophils. ECs cultures were isolated for the first time as early as in 1963 (2), in order to study isolated ECs response (3). In direct contact with Borrelia burgdorferi sensu lato (Bb sl.), the causative agent of Lyme disease, human umbilical vein endothelial cell (HUVEC) cultures are activated and demonstrate upregulation in the expression of some AMs. Such AMs are E-selectin, intracellular AM (ICAM-1), and vascular cell adhesion molecule (VCAM-1), which are regarded as godd markers of endothelial cell inflammatory response (4). In Slovenia, we successfully isolated primary HUVEC cultures for the first time in 1997. Results of optical density (OD) measurements of the expression of E-selectin and VCAM-1 on HUVEC cultures, when in contact with spirochete Bb sl., in compare with inflammatory cytokine tumor necrosis factor (TNF-alpha or cachectin), are described.
Descriptors     BORRELIA BURGDORFERI
VASCULAR CELL ADHESION MOLECULE-1
E-SELECTIN
IMMUNOHISTOCHEMISTRY
ENDOTHELIUM
UMBILICAL VEINS