Author/Editor		Debeljak, Nataša; Horvat, Simon; Komel, Radovan; Rozman, Damjana
Title		Molecular cloning and partial characterization of the mouse CYP51 cDNA
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 439, št. 3 Suppl
Publication year		2000
Volume		str. R7-8
Language		eng
Abstract		Northern analysis of the mouse testis and liver RNA show two types of lanosterol 14 alpha-demethylase (CYP51) transcripts, somatic and a testis-specific transcript. At least two CYP51 mRNAs are expressed in liver and testis, while the smallest transcript is expressed only in testis. The partial mouse Cyp51 cDNA (1052 bp) was obtained by RT-PCR cloning and was also found in expressed sequence tag (EST) clones. Bacterial artificial chromosome (BAC) clones containing mouse Cyp51 gene were also found. The coding part of the partial mouse CYP51 is 93.9% homologous to the rat and 87.9% to the human CYP51 cDNAs. There is less than 2% variation between the CYP51 EST clones to the RT-PCR cloned partial cDNA and BAC clones.
Descriptors		DNA, COMPLEMENTARY CLONING, MOLECULAR TESTIS LIVER LANOSTEROL MICE CHOLESTEROL TRANSCRIPTION, GENETIC POLYMERASE CHAIN REACTION