| Author/Editor | Battelino, Tadej; Miralles, Francisco; Kržišnik, Ciril; Scharfmann, Raphael; Czernichow, Paul | |
| Title | TGF-beta activates genes identified by differential mRNA display in pancreatic rudiments | |
| Type | članek | |
| Source | Pflugers Arch | |
| Vol. and No. | Letnik 439, št. 3 Suppl | |
| Publication year | 2000 | |
| Volume | str. R26-8 | |
| Language | eng | |
| Abstract | The effect of TGF-beta on gene activation in embryonic pancreatic rudiments was investigated using differential mRNA display. Several cDNA bands were augmented and some were suppressed in the presence of TGF-beta. Differentially expressed cDNAs were re-amplified, sequenced, and sequences compared to the GeneBank database. Glucagone and brain alpha-tropomyosin cDNA were identified from the group of augmented cDnas, andB-carboxypeptidase form the group of suppressed cDNAs. PCR experiments were confirmed with Northern blots. Obtained results are in accordance with immunohistochemical findings and render differential mRNA display a useful technique in identifying differentially expressed genes in embrionic pancreatic rudiments. Several unknown differentially expressed cDNA sequences obtained in our experiments remain to be identified. | |
| Descriptors | PANCREAS TRANSFORMING GROWTH FACTOR BETA RNA, MESSENGER GLUCAGON BLOTTING, NORTHERN RATS, WISTAR POLYMERASE CHAIN REACTION |