Author/Editor		Zajc-Kreft, Katarina; Kreft, Samo; Komel, Rado; Grubič, Zoran
Title		Nonradioactive northern blotting for the determination of acetylcholinesterase mRNA. Comparison to the radioactive technique
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 439, št. 3 Suppl
Publication year		2000
Volume		str. R66-7
Language		eng
Abstract		A sensitive nonradioactive northern blotting for the detection of acetylcholinesterase mRNA in mammalian tissues is described and compared to its radioactive version. Best results were obtained if digoxigenin labeled RNA probe was used for hybridization and CDP-StarTM, a chemiluminescent alkaline phosphatase substrate, for detection. The described nonradioactive technique for acetylcholinesterase mRNA determination is as sensitive as the radioactive one, but requires no protection against radiation and is less time consuming. Because of higher stability of the labeled probe, nonradioactive technique is also more convenient from the standpoint of experimental planning.
Descriptors		ACETYLCHOLINESTERASE RNA, MESSENGER MUSCLE, SKELETAL DIGOXIGENIN RATS BLOTTING, NORTHERN RNA PROBES