Author/Editor		Bedina-Zavec, Polona; Comino, Aleksandra; Watt, Paul; Komel, Radovan
Title		Interaction trap experiment with CDC6
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 439, št. 3 Suppl
Publication year		2000
Volume		str. R94-6
Language		eng
Abstract		CDC6 is an essential gene of yeast Saccharomyces cerevisiae. Although DNA sequence of the gene is available for a long time, biochemical function of Cdc6 protein in the cell cycle remains unclear. Using the interaction trap experiment we were looking for proteins interacting specifically with Cdc6. Four gene products interacting with Cdc6 were detected. By sequence analysis we found that ECMl1 codes for the protein involved in the cell wall synthesis, YNL201 codes for the protein of unknown function, probably involved in the carbon metabolism, YOR279 codes for protein of completely unknown function with no significant similarity with any known protein, and the interaction with Ty1 retrotransposition element was also found. The strongest interaction with Cdc6 bait measured as beta-galactosidase activity was observed with ECMlI and YNL20l; YOR279 interacts slightly weaker. The weakest beta-galactosidase activity was obtained by TylA element. The strongest suppression of cdc6-l mutation was observed by Ty1A element, the slight one with ECMl1 and YNL201 but no suppression of thermosensitive mutation was detected for YOR279.
Descriptors		SACCHAROMYCES CEREVISIAE CELL CYCLE PROTEINS GENES, CDC PLASMIDS BETA-GALACTOSIDASE HYBRIDIZATION