Author/Editor		Sušnik, Simona; Snoj, Aleš; Dovč, Peter
Title		Ponovitve dinukleotidnih in trinukleotidnih motivov v genomu lipana (Thymallus thymallus, L.)
Translated title		Dinucleotide and trinucleotide repeats in grayling (Thymallus thymallus, L.)
Type		članek
Source		Acta Biol Slov
Vol. and No.		Letnik 42, št. 3
Publication year		1999
Volume		str. 23-9
Language		slo
Abstract		The Soča river basin is a home of grayling (Soča-type) which is morphologically different from grayling in the Sava river drainage (Sava-type). As a consequence of the introduction of the Sava-type grayling into the Soča river system, the Soča-type grayling becomes seriously endangered in Slovenia. The application of genetic markers would enable more reliable differentiation between these two phenotipically distinct populations at the genomic level. Due to the lack of molecular markers for differentiation between geographically remote and morphologically distinct populations of grayling we prepared a partial genomic library of grayling and isolated clones containing microsatellite DNA markers. Within the cloned genomic fragments with a total length of 1150 kb,103 dinucleotide microsatellite loci were found. In addition, screening of genomic fragments in the total length of 2000 kb revealed three trinucleotide microsatellite loci. The average appearance of microsatellite loci with repeated dinucleotide CA and AG motifs in the grayling genome was estimated to be higher than in the other vertebrate genomes. The repeated dinucleotide motifs can be expected every 11.2 kb in the grayling genome. The trinucleotide motif CAA is less frequent and can be on average expected every 670 kb.
Summary		V soškem porečju živi lipan (soški tip), ki se morfološko razlikuje od lipana v savskem porečju (savski tip). Zaradi vnosa savskega tipa lipana v soško porečje je postal soški tip lipana ena izmed naših najbolj ogroženih populacij rib. Uporaba genetskih markerjev bi omogočila zanesljivejšo opredelitev teh dveh fenotipsko različnih populacij na ravni genomskih razlik. Ker molekulskih markerjev, s katerimi bi lahko razlikovali med tema dvema geografsko ločenima in morfološko različnima populacijama lipana, še ni bilo na voljo, smo izdelali delno genomsko knjižnico lipana in iz nje izolirali klone z mikrosatelitskimi lokusi. Na kloniranih fragmentih genoma s skupno dolžino 1150 kb smo našli 103 dinukleotidne mikrosatelite, ob pregledu večjega števila klonov s skupno dolžino 2000 kb pa še tri trinukleotidne mikrosatelitske lokuse. Ugotovili smo, da ocena števila lokusov s ponovitvami dinukleotidnih CA in AG motivov v genomu lipana presega pogostnost pojavljanja teh motivov pri drugih vretenčarjih. Ocenjujemo, da ponovitve dinukleotidnih motivov lahko pričakujemo vsakih 11.2 kb v genomu lipana. Trinukleotidni motiv CAA je precej redkejši, v povprečju se pojavlja enkrat na vsakih 670 kb.
Descriptors		SALMONIDAE GENE LIBRARY MICROSATELLITE REPEATS DINUCLEOTIDE REPEATS TRINUCLEOTIDE REPEATS