Author/Editor | Rozman, P; Drabbels, J; Schipper, RF; Doxiadis, I; Stein, S; Claas, FH | |
Title | Genotyping for human platelet-specific antigens HPA-1, -2, -3, -4 and -5 in the Slovenian population reveals a slightly increased frequency of HPA-1b and HPA-2b as compared to other European populations. | |
Type | članek | |
Source | Eur J Immunogenet | |
Vol. and No. | Letnik 26, št. 4 | |
Publication year | 1999 | |
Volume | str. 265-9 | |
Language | eng | |
Abstract | Typing of human platelet alloantigens (HPA) is necessary in various clinical situations. The purpose of this study was to type a random sample of the Slovenian population for HPA alleles, in order to obtain genetic population data. A total of 152 unrelated Slovenian blood donors were genotyped for HPA-1, -2, -3, -4 and -5 alleles using a simple method that enables simultaneous and complete determination of HPA genotypes. Ten different polymerase chain reactions employing sequence-specific priming (PCR-SSP), which worked in identical cycling conditions, were used. The allele frequencies were 0.809 for HPA-1a, 0.191 for HPA-1b, 0.891 for HPA-2a, 0.109 for HPA-2b, 0.591 for HPA-3a, 0.407 for HPA-3b, 0.997 for HPA-4a, 0.00 for HPA-4b, 0.934 for HPA-5a and 0.066 for HPA-5b. When compared to results of studies of various other Caucasian populations, our population displayed a slightly but not significantly higher proportion of the HPA-1b and 2b alleles. | |
Descriptors | ANTIGENS, HUMAN PLATELET IMMUNOPHENOTYPING GENE FREQUENCY GENOTYPE HOMOZYGOTE MICROSATELLITE REPEATS POLYMERASE CHAIN REACTION POLYMORPHISM (GENETICS) SEQUENCE ANALYSIS SLOVENIA |