| Author/Editor | Bavec, A; Jureus, A; Cigić, B; Langel, U; Zorko, M | |
| Title | Peptitergent PD1 affects the GTPase activity of rat brain cortical membranes | |
| Type | članek | |
| Source | Peptides | |
| Vol. and No. | Letnik 20, št. 2 | |
| Publication year | 1999 | |
| Volume | str. 177-84 | |
| Language | eng | |
| Abstract | Peptitergent PD1 shows complex effects on GTPase activity of rat brain cortical membranes: inhibition in the presence of lower concentrations of GTP and activation at a higher concentration, above 0.5 microM, of GTP. Its effect is dose dependent and is characterized by an EC50 of 1.8 +/- 0.2 microM and a Hill coefficient of 1.6 +/- 0.3, and it increases both Km and Vmax of the GTP hydrolysis. PD1 that was unable to solubilize G-proteins from the membranes probably acts on them by direct binding near the C-terminal alpha-helical region of the Galpha subunit, similarly to mastoparan. | |
| Descriptors | CEREBELLAR CORTEX DETERGENTS GTP PHOSPHOHYDROLASE MEMBRANE PROTEINS PEPTIDES AMINO ACID SEQUENCE GUANOSINE TRIPHOSPHATE GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE) HYDROLYSIS MICELLES MODELS, CHEMICAL MOLECULAR SEQUENCE DATA PROTEIN STRUCTURE, SECONDARY RATS, WISTAR SOLUBILITY WASP VENOMS |