Author/Editor		Kobe, Jasna Modrica
Title		Laboratorijska diagnostika malarije
Translated title		Laboratory diagnosis of malaria
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 38, št. Suppl 8
Publication year		1999
Volume		str. 145-8
Language		slo
Abstract		As we are preparing to enter the 21st century, the laboratory techniques of the 19th century remain in routine use and countinue to be the gold standard: microscopic examination of the Giemsa stained thick blood film for diagnosing malaria ( and other blood parasites) and the Pappenheim stained thin blood film plasmodium parasites in the peripheral blood and the determination of their quantity and species. The detection of a small percentage of parasite cells requires a high quality microscope and an experiencedmicroscopist. A new technology is available nowadays for low level infections: the Quantitative Buffy Coat method (QBC), wigh is based on the hematocrit principle, the fluorochrome acridine orange dye for the DNA and RNA of the Plasmodium and fluorescent mictoscopy. A rapid dipstick antigen capture assay is used for travellers; the strip is impregnated with specific monoclonal antibodies that bind to the histidine rich protein 2 (HRP-2) of Plasmodium falciparum or plasmodium specific glycolytic enzyme lactate dehydrogenase (pLDH) in the plasma.
Summary		Čeprav se pripravljamo na vstop v 21. stoletje, ostaja še naprej zlasti standard laboratorijska tehnologija 19. stoletja: mikroskopski pregled z Giemso obarvane goste (debele) kaplje za dokaz prisotnih malarijskih ali drugih krvnih parazitov ter po Pappenheimu obarvanega krvnega razmaza za določitev razvojnih stopenj, količine in vrste plazmodijskega parazita. Dokaz majhnega deleža s paraziti okuženih eritrocitov zahteva kokovosten mikroskop in izkušenega mikroskopista. Lažje jih dokažemo s koncentracijo parazitov na način, podoben določanju hematokrita, ob prisotnosti akridin orančnega barvila in njegove fluorokromne privlačnosti do DNA in RNA v parazitu, s pomočjo fluorescentne mikroskopije. Popotniki lahko uporabijo kot presejalni test testnetrakove, impregnirane s specifičnimi monoklonalnimi protitelesi, ki reagirajo s histidinom bogato beljakovino HRP-2 iz trofozoitov P. falciparum ali beljakovino pLDH, aktivnim plazmodijskim glikotičnim encimom.
Descriptors		MALARIA PLASMODIUM FALCIPARUM PLASMODIUM VIVAX PLASMODIUM MALARIAE DIAGNOSTIC TESTS, ROUTINE