Author/Editor		Benedičič, Desimira; Ravnikar, Maja; Kovač, Maja
Title		Auxins in bean (Phaseolus vulgaris L. Cv. Zorin) seedlings
Type		članek
Source		Phyton-Ann Rei Bot A
Vol. and No.		Letnik 39, št. 3 Spec Iss
Publication year		1999
Volume		str. 79-84
Language		eng
Abstract		The amount and composition of auxins were investigated in bean seedlings (Phaseolus vulgaris L.) cv. Zorin. Extracts of cotyledon, leaves, stems with shoots and roots were purified with Sep-Pak C18 cartridges and analysed by HPLC with fiuorescent and photodiode array detectors. Biological activity was determined with an Avena bioassay. The highest amount of indole-3-acetic acid (IAA) was detected in roots. In all analysed tissues bound IAA predominated. HPLC and bioassay confirmed IAA conjugates after hydrolysis with 7 N NaOH. In been seedlings, in addition to free IAA, some conjugates also showed biological activity.
Descriptors		LEGUMES SEEDS AUXINS GERMINATION CHROMATOGRAPHY, HIGH PRESSURE LIQUID INDOLEACETIC ACIDS COTYLEDON