Author/Editor		Grebenc, Tine; Rupnik, Maja; Hacin, Janez; Janc, Miha; Kraigher, Hojka
Title		A contribution on the studies of actinorhizal symbioses of Alnus spp. in Slovenia
Type		članek
Source		Phyton-Ann Rei Bot A
Vol. and No.		Letnik 39, št. 3 Spec Iss
Publication year		1999
Volume		str. 207-11
Language		eng
Abstract		Alders (Alnus spp) can form multiple symbiosis with ectomycorrhizal fungi and nitrogen fixing bacteria from the genus Frankia. We have tried to isolate Frankia from root nodules of Alnus glutinosa and A. incana, to culture them in different culture media and inoculate the seedlings of A. glutinosa, A. incana and A.viridis. The root nodules were surface sterilized and cultured in a modified BAP medium (LI & al.1996), a combination of BAP and nutrient medium (BnKER & al. 1979) and in a liquid medium. No growth was observed, although a reference strain of Frankia sp. from a culture collection in Oregon did grow in the combined medium. For inoculation, crushed surface sterilized nodules and sterile two-week old seedlings of alders, grown on the medium PNS (fInCIN & al.1997), were used. The nodulation was observed on A. glutinosa and A. incana in two growth conditions: in growth pouches and in vitro. PCR amplification with specific primers was performed to confirm the presence of a part of nitrogenase coding genes and to confirm isolates as Frankia alni species/group. We plan to continue with a screening for growth conditions and molecular characterizalion of Frankia strains from Slovenia and with combined inoculations with ectomycorrhizal fungi.
Descriptors		SYMBIOSIS PLANTS BACTERIA SEEDS CULTURE MEDIA POLYMERASE CHAIN REACTION NITROGENASE