Author/Editor		Kreft, Samo; Pompe, Maruša; Ravnikar, Maja; Umek, Andrej; Štrukelj, Borut
Title		Accumulation rate of rutin is decreased after infection of susceptible potato cultivar with PVYntn
Type		članek
Source		Phyton-Ann Rei Bot A
Vol. and No.		Letnik 39, št. 3 Spec Iss
Publication year		1999
Volume		str. 259-64
Language		eng
Abstract		Potato virus Y NTN is a member of a Potyviridae family, which causes a great damage in the cultivation of potato (Solanum tuberosum). Many cultivars (for instance cv. Igor) are sensitive to the infection and some are resistant or tolerant (for instance cv. Sante and cv. Desiree, respectively). In our study we followed the changes in secondary metabolite profile after infection of potato plants of the three cultivars. The plants were harvested 4 hours, 4 days and 14 days after infection. They were freeze-dried and extracted with methanol. The extracts were analysed by HPLC. By comparison of chromatograms of infected and control plants, we found only one peak, which differed significantly. The coresponding substance was later isolated and identified as rutin. The rutin content in the plants of all cultivars increases during the early development stage. The infection with PVY NTN inhibits this accumulation in susceptible potato cultivar (cv. Igor) by more than 50%, but it has no significant influence on the rutin accumulation in cv. Sante and cv. Desiree. There are two possible explanations for the observed phenomenon. Either the rutin has an active role in defence mechanism of potato plant, or its decreased accumulation is only due to redirection of biosynthetic pathways from rutin synthesis to the synthesis of other phenolic substances. To test this possibilities we grew infected plants in vitro in the medium containing.
Descriptors		POTATOES POTYVIRUS RUTIN CHROMATOGRAPHY, HIGH PRESSURE LIQUID ELECTROPHORESIS, CAPILLARY