Author/Editor		Stanič, Darja; Štrukelj, Borut; Štrucl, Renata; Demšar, Tina; Reavy, Brian; Barker, Hugh; Žel, Jana
Title		Transformation of Nicotiana tabacum cv. Samsun by the coat protein gene of PVYntn
Type		članek
Source		Phyton-Ann Rei Bot A
Vol. and No.		Letnik 39, št. 3 Spec Iss
Publication year		1999
Volume		str. 271-6
Language		eng
Abstract		Potato tuber necrotic ringspot disease, caused by a strain of potato virus Y (PVY NTN) has been reported in many countries in the last decade. It is one of the most harmful potato pathogens shown to have infected potato crops in Slovenia, cv. Igor being the worst affected. The long term aim of our study is to develop-virus-resistant transgenic lines of cv. Igor expressing pathogen-derived sequences. The tobacco plants (Nicotiana tabacum cv. Samsun) served as a model species for transformation and resistance studies. The coat protein (CP) gene of PVY NTN was cloned into a pUC plasmid and then into a pROK2 plasmid which was mobilised in Agrobacterium tumefaciens strain LBA 4404. The presence of CP gene in the regenerated plants was checked by PCR and all primary transformants tested were found to be transgenic. Inoculated plants of the transgenic tobacco lines were tested for resistance to PVY NTN but developed symptoms of PVY NTN and appear to have no virus resistance.
Descriptors		TOBACCO TRANSFORMATION, GENETIC CAPSID GENES, VIRAL POLYMERASE CHAIN REACTION PLANTS, TRANSGENIC