Author/Editor		Zorn, Branko; Virant-Klun, Irma; Drobnič, Sašo; Tomaževič, Tomaž; Talan, Tanja; Peterlin, Borut; Ravnik-Glavač, Metka; Meden-Vrtovec, H
Title		Nosečnost in otroci po metodi neposrednega vnosa semenčice v citoplazmo jajčne celice na Ginekološki kliniki v Ljubljani: retrospektivna študija ob rojstvu 101. otroka
Translated title		Pregnancies and children after intracytoplasmic sperm injection attempt at the Department of obstretics and gynecology in Ljubljana: retrospective study after the birth of 101 children
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 69, št. 2
Publication year		2000
Volume		str. 67-73
Language		slo
Abstract		Background. To analyze the pregnancy, obstetric outcome, and status of newborns conceived after intracytoplasmic sperm injection (ICSI) at the Department of obstetrics and gynaecology in Ljubljana. Methods. After ICSl, retrospective analysis of pregnancy rates obstetric outcome, and frequency of chromosomal abnormalities and congenital malformations was done in comparison with conventional in vitro fertilization (IVF) and general population. Data were obtained from the records of biologists, geneticsts obstetricians-gynecologists and/or pediatricians. Researched groups. Group 1: children, concieved after ICSl. A total of 419 couples in whom the male partners were presumed to be the cause of at least one failed IVF attempt or whose semen parameters were unacceptable for conventional IVF treatment underwent 687 ICSI cycles from july 1995 to june 1997. Group 2: children, conceived after IVF. During the same time period 1643 conventional IVF attempts were performed. Group 3:11,466 newborns from general populatiun were born at the Ljubljana's maternity in the two year time period 1995-1996. Results. In the two year period 107 patients became pregnant after ICSI. Clinical pregnancy per embryo transfer was 17%, with a resultant delivery rate of 11 % per cycle. In 23 women (21 %) pregnancy resulted in spontaneous abortion. In 7 of 23 miscarriages for which cytogenetic data were available, a trisomy and a tetraploidy were found. Miscarriage was correlated with higher age of the woman (> 40 years), poor sperm morphology, and immaturity of spermatozoa from testis or epididymis. (Abstract truncated at 2000 characters.)
Summary		Izhodišča. Oceniti potek in izid nosečnosti oz. stanje otrok, rojenih po zdravljenju neplodnosti s postopkom zunajtelesne oploditve z neposrednim vnosom semenčice v citoplazmo jajčne celice (ICSI) na Ginekološki kliniki v Ljubljani. Metode. Retrospektivno smo analizirali potek nosečnosti in poroda ter incidenco kromosomskih nenormalnosti in prirojenih napak pri otrocih, rojenih po metodi ICSI, v primerjavi z otroki, rojenimi po navadnem postopku zunajtelesne oploditve (NF), in z otroki, rojenimi v splošni populaciji. Podatke smo dobili iz poročil biologov genetikov, porodničarjev in pediatrov. Raziskovane skupine. Prva skupina: otroci, spočeti po metodi ICSI. Od julija 1995 do junija 1997 smo v 687 ciklusih zunajtelesne oploditve uporabili metodo ICSI pri 419parih zaradi moške neplodnosti ali vsaj enkrat neuspešnega poskusa navadne zunajtelesne oploditve. Druga skupina: otroci, spočeti po navadnem postopku zunajtelesne oploditve (NF). V istem obdobju smo izvedli 1643 ciklusov IVF Tretja skupina: 11.466 otrok, rojenih v letih 1995-1996 v splošni populaciji v ljubjanski porodnišnici. Rezultati. V obdobju dveh let je po uporabi metode ICSI zanosilo 107 žensk. Nosečnosti na prenos zarodka je bilo 17%, po- rodov na ciklus pa 11 %. Do spontanega splava je prišlo pri 23 ženskah (21 %). Pri 7 od 23 žensk, ki so spontano splavile, smo naredili citogenetsko analizo. Našli smo eno tetraploidijo in eno trisomijo. Starost matere nad 40 let, teratozoospermija kot vzrok moške neplodnosti in nezrelost semenčic iz nadmodka in moda so bili povezani z večjim deležem splavov. (Izvleček skrajšan na 2000 znakov.)
Descriptors		FERTILIZATION IN VITRO SPERM-OVUM INTERACTIONS ABORTION CHROMOSOME ABNORMALITIES INFANT, NEWBORN PREGNANCY OUTCOME ABNORMALITIES INFANT, NEWBORN, DISEASES