Author/Editor     Kopač, I; Sterle, M; Batista, U
Title     Vpliv kemičnih retrakcijskih sredstev na celične kulture keratinocitov dlesne
Translated title     The influence of chemical retraction agents on cell cultures of gingival keratinocytes
Type     članek
Source     Zobozdrav Vestn
Vol. and No.     Letnik 55, št. 1
Publication year     2000
Volume     str. 3-11
Language     slo
Abstract     Prior to fixed prosthodontic impression procedures, temporary horizontal retraction of the free gingival tissue should be accomplished apically to the preparation finishing line. The mechanical-chemical method using cotton retraction cords of various sizes, impregnated with various retraction chemicals, is the most commonly employed retraction technique. Most retraction agents have low pH values and are therefore hazardous to the cut dentine and periodontal tissues. Sympathomimetic vasoconstrictors introduced recently have more physiological pH values and are free of systemic side-effects. The aims of the study were: first, to culture gingival keratinocytes, and second, to observe and compare the effects of two different chemical retraction agents on the cultured cells. The most obvious differences were observed between control specimens and specimens treated with 25 % aluminum chloride: the treated specimens had smoother cell surfaces and destroyed intercellular contacts, compared to the control specimens. The cultures treated with 0.05% tetrahydrozoline showed only minor surface changes, compared to the untreated cultures. Despite incomplete terminal differentiation in cultures, keratinocytes represent a suitable model for testing chemical retraction agents. The results contribute to the proper selection of retraction agents for clinical use.
Descriptors     GINGIVA
KERATINOCYTES
RATS, WISTAR
CELLS, CULTURED
MICROSCOPY, ELECTRON
ALUMINUM
CHLORIDES
IMIDAZOLES
CULTURE MEDIA