Author/Editor | Pajer, Z; Coer, A; Štiblar-Martinčič, D | |
Title | Morphology of FRTL-5 cell colonies in a semi-solid medium | |
Type | članek | |
Source | J Endocrinol Invest | |
Vol. and No. | Letnik 23 | |
Publication year | 2000 | |
Volume | str. 218-2 | |
Language | eng | |
Abstract | For the first time, 3.7 and 10 days growth of normal thyroid follicular FRTL-5 cell colonies in a semi-solid medium of 0.6% methocel over 1% agar base was morphometrically analyzed. It was found that the areas of FRTL-5 colonies as well as their perimeters and maximum diameters increased, while according to their form factors the FRTL-5 colonies were regular in shape. After 10 days in a semi-solid medium, 83% of the FRTL-5 colonies had maximum diameters greater than 50 um. It is obvious that after long culturing of FRTL-5 cells under the influence of the pituitary thyroid-stimulating hormone (TSH) these cells are not uniform anymore and that they grow in a semi-solid medium. | |
Descriptors | THYROID GLAND CELL LINE THYROTROPIN CELL SIZE CULTURE MEDIA |