Author/Editor     Nagata, Tetsuji
Title     Three-dimensional observations on thick biological speciments by high voltage electron microscopy
Type     članek
Source     Image Anal Stereol
Vol. and No.     Letnik 19, št. 1
Publication year     2000
Volume     str. 51-6
Language     eng
Abstract     Thick biological specimens prepared as whole mount cultured cells or thick sections from embedded tissues were stained with histochemical reactions, such as thiamine pyrophosphatase, glucose-6-phosphatase, cytochrome oxidase, acid phosphatase, DAB reactions and radioautography, to observe 3-D ultrastructures of cell organelles producing stereo-pairs by high voltage electron microscopy at accerelating voltages of 400-1000 kV. The organelles demonstrated were Golgi apparatus, endoplasmic reticulurn, mitochondria, lysosomes, peroxisomes. pinocytotic vesicles and incorporations of radioactive compounds. As the results, those cell organelles were observed 3-dimensionally and the relative relationships between these organelles were demonstrated.
Descriptors     ORGANELLES
LIVER
MICROBODIES
THIAMINE PYROPHOSPHATASE
GLUCOSE-6-PHOSPHATASE
CYTOCHROME-C OXIDASE
ACID PHOSPHATASE
RATS, WISTAR
HISTOCYTOCHEMISTRY
MICROSCOPY, ELECTRON
GOLGI APPARATUS
ENDOPLASMIC RETICULUM
LYSOSOMES