Author/Editor		Milutinović, Aleksandra
Title		Vpliv mikrocistinov na regeneracijo jetrnih celic podgane
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2000
Volume		str. 64
Language		slo
Abstract		Microcystins are a family of over SO structurally similar cyanobacterial hepatotoxins. The toxins have been found in eutrophycated waters in all parts of Slovenia. The only exception are the mountain lakes at high altitude. In order to assess the presence of toxic cyanobacterial bloom in densely populated area a sample was taken from "Koseški bajer" in Ljubljana, and it was found that it contains microcystin YR, LR an RR. A cumulative toxicity of the toxins was assumed, and LD 50 was determined: for MC-YR 20.45 microg/kg for YR 55.76 microg/kg and for RR 11.15 microg/kg. The enlargement of liver mass was dose-dependent. Although acute toxic effects are highly lethal the chronic effects may be more important for human population due to the possible chronic exposure to sublethal doses of the toxins. Chronic effects have been studied on male rats (Fischer) after partial hepatectomy. Liver regeneration as well as possible degenerative effects were studied using MRI, patohystological techniques and macroscopic pathoanatomical observation. After the surgical liver resection one group of animals received a mixture of methanol and ethanol, and the other group received microcystin YR (5 microg/kg) dissolved in the mixture of methanol and ethanol. Weight gain and food consumption were also measured throughout the experiment. The weight gain was reduced in the group treated with MC-YR, and liver regeneration was suppressed three fold as compared to the control group. Macroscopic pathoanatomical inspection also revealed impaired liver regeneration in the group of experimental animals treated with MC-YR. Pathohystological examination confirmed these findings. Additionally, degenerative changes of the liver left after the surgical resection were observed both in the control group treated with alcohol and in the group treated with microcystins, but the degeneration was more pronounced in the latter. (Abstract truncated at 2000 characters).
Descriptors		LIVER DISEASES BACTERIAL TOXINS ALCOHOL, ETHYL LIVER REGENERATION RATS MICE CYANOBACTERIA WATER POLLUTANTS FRESH WATER MAGNETIC RESONANCE IMAGING LETHAL DOSE 50