Author/Editor		Zore, Irena
Title		Določanje kompleksa katepsin B / cistatin C v bioloških vzorcih in njegova vloga pri napredovanju raka
Translated title		Detection of cathepsin B / cystatin C complex in biological samples and its role in cancer progresion
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2000
Volume		str. 73
Language		slo
Abstract		A sandwich-type ELISA was developed for assessment of a complex between cathepsin B (CB) and cystatin C (CC) in sera. The assay is based on a combination of two monoclonal antibodies, first one (3E1) - catching Ab, was raised against CB and the second one (1A2)-detecting Ab, conjugated with horseradish peroxidase, against CC. Only CB/CC complex, cross-linked and non cross-linked, was able to evoke the signal in ELISA. Detection limit of the assay was 31.25 nmol/1 and the working range between 31.3 - 400 nmol/1. The within and between-run coefficient of variance (CV) varied from 4.7% to 9.4% and from 11 % to 12.8%, respectively, indicating satisfactory reproducibility of the method. The content of CB/CC complex was determined in sera of 133 healthy controls, 32 patients with benign lung diseases, 148 with lung and 32 with colorectal cancer. CB/CC complex was significantly less abundant in sera of patients bearing malignant lung and colorectal tumors than in those with benign lung diseases or healthy controls (p<0.001). In colorectal cancer sera its level was significantly higher in early Dukes' stages A and B than in advanced stages C and D (p=0.02). Our results support the hypothesis of hindered inhibitory capability between KB and CC during cancer progression.
Descriptors		CATHEPSIN B CYSTATINS LUNG NEOPLASMS COLORECTAL NEOPLASMS NEOPLASM STAGING NEOPLASM INVASIVENESS HYBRIDOMAS ENZYME-LINKED IMMUNOSORBENT ASSAY ANTIBODIES, MONOCLONAL HORSERADISH PEROXIDASE