Author/Editor		Popović, M; Bresjanac, M; Sketelj, J
Title		Role of axion-deprived Scwann cells in perineurial regeneration in the rat sciatic nerve
Type		članek
Source		Neuropathol Appl Neurobiol
Vol. and No.		Letnik 26
Publication year		2000
Volume		str. 221-31
Language		eng
Abstract		The role of Schwann cells (SC) in perineurial regeneration after nerve injury has not yet been resolved. It was hypothesized that SC alone are able to induce at least partial morphological restoration of the destroyed orthotopic perineureum (PN). To test the hypothesis, a permanently denervated segment of the rat sciatic nerve was made acellular by freeze-thawing, except in its most proximal part where non-neuronal cells were left intact. Restoration of the frozen segment by these cells was examined by electron microscopy and immunohistochemistry of the SC marker, S-100 protein, 4 and 8 weeks after injury. The PN regenerated from undifferentiated fibroblast-like cells. In the presence of migrant SC without axons, regenerated cells in the place of the former PN were stacked in several layers and, in accordance with the hypothesis, partially expressed typical features of the perineurial cells (PC): pinocytotic vesicles, short fragments of basal lamina and tight junctions. Migrant SC induced formation of pseudo-minifascicles even in the epineurium. In these, SC organized the adjacent fibroblasts into a multilayered circular sheath, and induced their partial differentitation towards perineurial cells. Further experiments demonstrated that regenerating axons are required for complete morphological differentation of the regenerated perineurial cells either in the orthotopic PN or in minifascicles.
Descriptors		SCIATIC NERVE NERVE REGENERATION SCHWANN CELLS RATS, WISTAR AXONS MICROSCOPY, ELECTRON IMMUNOHISTOCHEMISTRY