Author/Editor		Malovrh, Tadej; Škoberne, Mojca; Gruntar, Igor; Kotnik, Vladimir
Title		The cell-mediated immune response to Borrelia afzelii, garinii and bugdorferi in C57BL/6 mice is dependent on antigen specificity
Type		članek
Source		Fems Immunol Med Microbiol
Vol. and No.		Letnik 28
Publication year		2000
Volume		str. 233-40
Language		eng
Abstract		Inbred C57BL/6 mice were challenged with Borrelia afzelii. Borrelia garinii and Borrelia burgdorferi sensu stricto and tested for antigen-specific T-cell response in vitro. The sonicated preparations of in vitro grown spirochetes were capable of stimulating polyclonal proliferation and specific cell-mediated response. depending on duration of the cell culture. Murine splenocytes previously sensitized to B. burgdorferi sensu lato (s.l.), but not those from control mice be induced for antigen-specific proliferation in vitro. Moreover, detectable cell-mediated response could be induced only with antigen preparations derived from a corresponding strain but not with those obtained from other Borrelia genospecies as revealed by the (3H)thymidine incorporation assay. The current study considers that the strict B. burgdorferi s.l. antigen-specific response may also be expected in infections in humans and contributes to the explanation of the frequently poor antibody- and cell-mediated immune response observed in patients diagnosed with Lyme disease.
Descriptors		BORRELIA LYMPHOCYTE TRANSFORMATION ANTIBODIES, BACTERIAL MICE, INBRED C57BL BORRELIA BURGDORFERI