Author/Editor		Simčič, Saša; Wraber, Branka; Sollner, Marja; Urleb, Uroš; Gobec, Stanislav
Title		Modulation of tumour necrosis factor production with desmuramyldipeptide analogues
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 440, št. 5 Suppl
Publication year		2000
Volume		str. R64-6
Language		eng
Abstract		Some synthetic analogues of the immunomodulatory agent muramyl dipeptide (MDP), i.e. phthalimido- (LK-511, LK-413, LK-512, LK-423, LK-508), adamantyl- (LK-415, LK-517), 7-oxoalkyl-(LK- 409) desmuramylpeptides were assessed for the tumour necrosis factor (TNF) inducing activity and the ability to modulate TNF production in in vitro phorbol 12-myristate 13-acetate (PMA) & ionomycin stimulated cultures of human peripheral blood mononuclear cells. A kinetic study over a 40-hour period indicated that desmuramyldipeptides were weak TNF inducers compared to romurtide, PMA & ionomycin or lipopolysaccharide. By contrast, they showed the potential to up- or down-regulate the production of TNF evoked by PMA & ionomycin, which was strongly dependent on the time of the stimulation. After 4h of stimulation, the TNF secretion was augmented by LK-508, LK-409 and LK- 511, after 18 h by LK-409 and LK-423, and after 40 h by LK-423, LK-511, LK-415 and LK-512. However, LK-517 and LK-512 inhibited the secretion of TNF after the 18-h period.
Descriptors		LEUKOCYTES, MONONUCLEAR LYMPHOCYTE TRANSFORMATION TUMOR NECROSIS FACTOR ACETYLMURAMYL-ALANYL-ISOGLUTAMINE IONOMYCIN TETRADECANOYLPHORBOL ACETATE KINETICS