| Author/Editor | Bavec, Aljoša; Podgornik, Aleš; Zorko, Matjaž | |
| Title | Purification of GTPgammaS binding proteins from membranes of porcine brain using convective interaction media (CIM) supports | |
| Translated title | Čiščenje proteinov, ki vežejo GTPgamaS, iz membran prašičjih možgan z uporabo CIM nosilcev | |
| Type | članek | |
| Source | Acta Chim Slov | |
| Vol. and No. | Letnik 47, št. 3 | |
| Publication year | 2000 | |
| Volume | str. 371-9 | |
| Language | eng | |
| Abstract | Membranes from porcine brain bind relatively large quantities of guanosine 5'-(3-0-thio) triphosphate (GTPgammaS), a poorly-hydrolysable analogue of GTP. Chrotnatography of solubilized proteins with 1% Sodium cholate using CIM DEAE column (Convective Interaction Media) revealed two fractions reach in GTPgammaS binding proteins. In reduced SDS-PAGE analysis only these two fractions showed a protein band with molecular mass 42 kDa which corresponded to alpha subunits Gs Gi or G11. Comparison between CIM DEAE and traditional DEAE-Sephacel methods showed that membrane bound heterotrimeric G - proteins might be isolated by HPLC using CIM supports on second or minute time scale. | |
| Descriptors | BRAIN CELL MEMBRANE GTP-BINDING PROTEINS GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE) SWINE CHROMATOGRAPHY, DEAE-CELLULOSE CHROMATOGRAPHY, HIGH PRESSURE LIQUID ELECTROPHORESIS, POLYACRYLAMIDE GEL |