Author/Editor     Zima, T; Tesar, V; Crkovska, J; Stejskalova, A; Teminova, J; Nemeček, K; Štipek, S; Platenik, J; Janebova, M
Title     Cardioxane-ICFR-187 protects from adriamicyn-induced nephrotic syndome
Type     članek
Source     In: Lindič J, Kaplan-Pavlovčič S, editors. Zbornik prispevkov 1. slovenski nefrološki kongres z mednarodno udeležbo; 1996 okt 23-26; Portorož. Ljubljana: Klinični center, Nefrološka klinika,
Publication year     2000
Volume     str. 44-7
Language     eng
Abstract     Reactive oxygen species produced during metabolism of adriamycine (ADR) are supposed to play an important role in the pathogenesis of experimental adriamycine nephropathy in rats. Cardioxane (ICRF - 187), an iron chelator, has been shown to inhibit adriamycine-induced formation of hydroxyl radical. Cardioxane (CAR) also decreased adramycine cardiotoxicity in oncological patients. Aim of our study was to assess the putative role of cardioxane in adriamycine nephropathy and so to evaluate the possible participation of free radicals in its pathogenesis. We examined 5 experimental groups A (adriamycine given as a single dose 5 mg/kg bw i.v.), CA (CAR given as a single dose 100 mg/kg bw before ADR administration), CCA (the same as CA, but CAR applied moreover i.p. weekly 100 mg/kg bw), C (rats given only CAR 100 mg/kg bw i.p. weekly), N (rats given only i.v. saline - controls). Common biochemical parameters, malondialdehyde (MDA) using HPLC and antioxidant enzymes (glutathione peroxidase - Gpx and superoxide dismutase - SOD) in blood and kidney homogenates and also histology of the kidney, heart and blood marrow were studied after the rats were sacrificed. Full-blown nephrotic syndrome (NS) developed after 3 weeks only in A rats. NS was completely prevented in all CAR treated rats (CA, CCA). Proteinuria was significantly increased in A rats (108.2 +- 48.4 mg/L of GF in comparison with CA 12.4 +- 6.8mg/Lp<0.001) and with N (6.1 +- 3.5 mg/Lp < 0.0001). Total MDA in erythrocytes was significantly increased only in A rats (1.7 +- 0.3 micromolol/L) and was completely normalized by CAR in CA (1.1 +- 0.2 micromol/L, p < 0.001). Activity of GPx in kidney homogenate and in erythrocytes was not significantly increased by CAR in adriamycine treated rats. (Abstract truncated at 2000 characters.)
Descriptors     NEPHROTIC SYNDROME
DOXORUBICIN
IRON CHELATES
PROTEINURIA
RATS
CREATININE
MALONDIALDEHYDE
CHROMATOGRAPHY, HIGH PRESSURE LIQUID