Author/Editor		Čala, S; Kordić, D; Pavlović, D
Title		Monocytes in haemodialysis patients overproduce nitric oxide
Type		članek
Source		In: Lindič J, Kaplan-Pavlovčič S, editors. Zbornik prispevkov 1. slovenski nefrološki kongres z mednarodno udeležbo; 1996 okt 23-26; Portorož. Ljubljana: Klinični center, Nefrološka klinika,
Publication year		2000
Volume		str. 253-6
Language		eng
Abstract		Human monocytes do not secrete large cytotoxic amounts of nitric oxide (NO) as some animal monocytes do, but low levels produced are considered to be important regulator of immune and inflammatory processes. Multiple monocyte abnormalities in haemodialysis patients excited our interest in examining monocyte NO synthesis. In 7 uraemic patients treated by haemodialysis, production of NO by peripheral blood monocytes was measured, and compared to NO production by monocytes in 9 healthy coritrols. In uraemic patients, blood was taken before haemodialysis. Monocytes were isolated by Lymphoprep centrifugation and glass adherence. They were incubated for 18 hours in the medium (RPMI + 10 % calf serum) containing 10 (g/ml endotoxin. NO released in the medium was measured as nitrite ion by the Griess method. Monocytes isolated from the peripheral blood of haemodialysis patients produced 6.2 microM NO (median, range 2.3-29.3) during incubation. Production in control monocytes was 1.4 microM NO (0.4-7.4), significantly less than in haemodialysis patients' monocytes (p = 0.006 by Mann-Whitney U test). In haemodialysis patients, monocytes secrete five times more NO compared to controls. Monocyte NO overproduction in uraemia could be the cause of the disturbance of cellular and humoral immunity.
Descriptors		HEMODIALYSIS MONOCYTES NITRIC OXIDE UREMIA