Author/Editor     Gomišček, Gregor; Arrigler, Vesna; Gros, Marjan; Zupančič, Mirjana; Svetina, Saša
Title     Asymmetrical labeling of giant phospholipid vesicles
Type     članek
Source     Pflugers Arch
Vol. and No.     Letnik 440, št. 5 Suppl
Publication year     2000
Volume     str. R51-2
Language     eng
Abstract     The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50% decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles.
Descriptors     FLUOROMETRY
PHOSPHOLIPIDS
MEMBRANES, ARTIFICIAL
DITHIONITE
MICROSCOPY, FLUORESCENCE