Author/Editor | Gomišček, Gregor; Arrigler, Vesna; Gros, Marjan; Zupančič, Mirjana; Svetina, Saša | |
Title | Asymmetrical labeling of giant phospholipid vesicles | |
Type | članek | |
Source | Pflugers Arch | |
Vol. and No. | Letnik 440, št. 5 Suppl | |
Publication year | 2000 | |
Volume | str. R51-2 | |
Language | eng | |
Abstract | The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50% decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles. | |
Descriptors | FLUOROMETRY PHOSPHOLIPIDS MEMBRANES, ARTIFICIAL DITHIONITE MICROSCOPY, FLUORESCENCE |