Author/Editor		Škoberne, Mojca; Malovrh, Tadej; Skralovnik-Štern, Aleksandra; Kotnik, Vladimir
Title		Human peripheral blood lymphocytes sensitised to PPD respond to in vitro stimulation with increased expression of CD69 and CD134 activation antigens and production of Th-1 type cytokines
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 440, št. 5 Suppl
Publication year		2000
Volume		str. R58-60
Language		eng
Abstract		Individuals sensitised to Mycobacterium tuberculosis antigens by infection, vaccination or Mantoux test generate specific memory cells. The response to in vitro restimulation with PPD is observed as the lymphoid cell proliferation and production of Th-1 type cytokines. Cell-mediated immune response was measured by Mantoux test, lymphocyte blast transformation test, estimation of IFN- gamma production (Quantiferon ELISPOT), and expression of CD69 and CD134 molecules on the T-helper lymphocytes (CD4+). All the methods used were compared for parity of the results According to Mantoux test results, the patients could be distributed into two groups: responder and non-responder group. Induration in Mantoux test after a new contact with PPD in non-responders was smaller than 5 nmm, they produced only small amounts of IFN-gamma, lymphocyte blast transformation was poor and expression of CD69 and CD134 was low. In responders reaction was much more intensive in all tests measured. We conclude that the reactivity of memory cells to M tuberculosis antigens can be effectively detected by Mantoux test. The scone was true also for the In vitro tests presented but in addition the in vitro tests give more information about the mechanism involved in the immune response against M. tuberculosis.
Descriptors		MYCOBACTERIUM TUBERCULOSIS TUBERCULIN TEST LYMPHOCYTE TRANSFORMATION INTERFERON TYPE II T-LYMPHOCYTES T-LYMPHOCYTES, HELPER-INDUCER FLOW CYTOMETRY CYTOKINES