Author/Editor		Galvani, Vesna; Pretnar-Hartman, Katrina; Rupreht, Ruth R; Novaković, Srdjan; Štabuc, Borut; Ocvirk, Janja; Menart, Viktor; Gaberc-Porekar, Vladimira; Štalc, Anton; Rožman, Primož; Čurin-Šerbec, Vladka
Title		Soluble tumor necrosis factor receptor I (sTNFRI) as a prognostic factor in melanoma patients in Slovene population
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 440, št. 5 Suppl
Publication year		2000
Volume		str. R61-3
Language		eng
Abstract		Tumor necrosis factor a (TNF-alpha) and its receptors (TNFRI and TNFKII) which exist in soluble form as a product of cleavage of the extracellular domain of mebrane integrated receptors, still rise debate about their importance. It was reported that TNF-alpha has numerous actions in diseases such as inflammation, autoimmunity, infectious diseases, septic shock and many types of cancer (1, 2). Several authors have reported the significance of sTNFRI level in serum of cancer patients (3, 4). This study was performed in collaboration with the Institute of Oncology of Slovenia. At least two different mouse monoclonal antibodies (MAbs) against human sTNFRI have been prepared to obtain a sensitive and reliable sandwich ELISA. It was compared with commercially available R and D and Endogen ELISAs for the determination of sTNFRI. Groups of patients with different stages of melanoma and epithelial ovarian carcinoma were tested and their clinical records were reexamined. Levels of sTNFRI were measured and compared with the normal serum levels of sTNFRI.
Descriptors		TUMOR NECROSIS FACTOR MELANOMA RECEPTORS, TUMOR NECROSIS FACTOR OVARIAN NEOPLASMS NEOPLASM STAGING PROGNOSIS