Author/Editor		Rupreht, Ruth Rebeka; Mozetič-Francky, Bojana; Francky, Andrej; Matis, Maja; Škobene, Mojca; Galvani, Vesna; Malovrh, Tadej; Kotnik, Vladimir; Čurin-Šerbec, Vladka
Title		Murine monoclonal antibodies direct against human recombinant macrophage migration inhibitory factor
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 440, št. 5 Suppl
Publication year		2000
Volume		str. R78-80
Language		eng
Abstract		Macrophage Migration Inhibitory Factor (MIF) is a crucial component of the immune system acting together with glucocorticosteroids to regulate immunity and inflammation Understanding of its many putative functions and action mechanisms is still ambiguous. Due to the newest findings that a local MIF expression is up regulated in allograft rejection and in glomerulonephritis, an interest in MIF research is increasing and is focused on possibilities of anti-MIF treatment. In the present work new marine monoclonal antibodies (MAbs) directed against human recombinant MIF (hrMIF) are described. hrMIF protein used for the immunisation was tested for its biological activity and has evident macrophage migration inhibitory activity. The selected MAbs were purified and further characterised. They recognised MIF in a Western blot experiment after a native IEF. Anti-MIF MAb designated as MI inhibited MIF activity in the test, which was performed in the 48 well Boyden chamber system. It is presumed that M1 MAb could be used as a potential therapeutic agent.
Descriptors		MACROPHAGE MIGRATION-INHIBITORY FACTORS ANTIBODIES, MONOCLONAL CELL MIGRATION INHIBITION MICE ENZYME-LINKED IMMUNOSORBENT ASSAY