Author/Editor		Kraševec, Nada; van den Hondel, Cees AMJJ; Komel, Radovan
Title		Expression of human lymphotoxin alpha in Aspergillus niger
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 440, št. 5 Suppl
Publication year		2000
Volume		str. R83-5
Language		eng
Abstract		A gene-fusion expression strategy was applied for heterologous expression of human lymphutoxin alpha (LTalpha) in the Aspergillus niger ABI.13 protease-deficient strain. The LTalpha gene was fused with the A. niger glucoamylase GII-form as a carrier-gene, behind its transcription control and secretion signals. Special attention was paid to the influence of different colon usage on secretion of protein. In the case of human tumor necrosis factor alpha (TNFalpha) a dramatic change of secretion has been observed when human cDNA sequence was used instead of synthetic E. coli biased codons. In the case of LTalpha such a change of codon usage brought improvement at the RNA level, however, no increase in the quantity of secreted protein was observed, due to the proteolitic activity of the host organism. The estimated yield of secretion of LTalpha from A. niger into the soya medium was 50 pg 1-1 of culture.
Descriptors		ASPERGILLUS NIGER CLONING, MOLECULAR LYMPHOTOXIN TUMOR NECROSIS FACTOR CODON TRANSFORMATION, GENETIC PROTEINS ELECTROPHORESIS, POLYACRYLAMIDE GEL IMMUNOBLOTTING