Author/Editor		Ihan, Alojz; Tepeš, Bojan; Gubina, Marija
Title		Diminished Th1-type cytokine production in gastric mucosa T-lymphocytes after H. pylori eradication in duodenal ulcer patients
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 440, št. 5 Suppl
Publication year		2000
Volume		str. R89-90
Language		eng
Abstract		Helicobacter pylori infects an estimated 50% of the world population, however only a small proportion of individuals develop clinical symptoms of gastritis, peptic ulceration or gastric cancer. The variations in disease presentation may be due to differences in bacterial virulence and/or immune response to the pathogen. In the previous study we reported an increased expression of the IL-2 receptor in duodenal ulcer (DU) patients infected with H. pylori. This study examined intracellular lymphokine production in gastric mucosa infiltrating T lymphocytes in DU patients before and after H. pylori eradication. T lymphocytes were isolated from gastric mucosa biopsies by using mechanical and enzymatic tissue desegregation Ficoll-purified lymphocytes were incubated with monoclonal antibodies and analysed by using 3-colour flow cytometry analysis for intracellular interferon gamma (IFNgamma) and interleukin 4 (IL-4) expression in order to define Th1 and Th2 cell population We demonstrated a significant decrease in the proportion of Th1 cells infiltrating gastric mucosa 6 and 12 months after H. pylori eradication. Our results suggest the importance of the local immune response in the development of H. pylori related gastritis.
Descriptors		DUODENAL ULCER HELICOBACTER PYLORI GASTRITIS T-LYMPHOCYTES CYTOKINES GASTRIC MUCOSA TH1 CELLS FLOW CYTOMETRY INTERLEUKIN-2 INTERLEUKIN-4 INTERFERON TYPE II