Author/Editor		Tomšič, Martin; Bajrović, Fajko
Title		Sensitivity of peripheral nerve fibres to sodium pentobarbital anaesthesia in rat
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 440, št. 5 Suppl
Publication year		2000
Volume		str. R107-8
Language		eng
Abstract		The sensitivity of different classes of peripheral nerve fibres to anaesthesia with sodium pentobarbital in rat was tested. The basic approach was to examine changes in compound action potential (CAP) in a rat sural nerve induced by sodium pentobarbital. Rats were either sacrified by cervical dislocation (control) or anaesthetized with sodium pentobarbital (100 mg/kg, i.p.), and a 30 mm long sural nerve segment excised and placed on electrodes in a thermostatically controlled recording chamber. CAPs were evoked by electrically stimulating the sural nerve segment with supra-threshold stimuli. CAP in control sural nerves consisted of the early component (A axons 32.73 +- 2.91 m/s) and the late component (C axons: 0.92 +- (0.05 m/s) with peak voltage amplitudes 4.9 +- 1 0 mV and 0.1 +- 0.03 mV, respectively. Anaesthesia with sodium pentobarbital had no effect on CAP latency. However, the amplitude of CAP of both A and C axons was reduced by approximately 40 % and 50 %, respectively.The depressant effect of pentobarbital on CAPs was statistically significant for both groups of axons (p<0.01). Non-selective sensitivity of A and C axons to pentobarbital suggests even distribution of receptors for GABA in these two populations of axons in the rat peripheral nerve.
Descriptors		SURAL NERVE ANESTHESIA, GENERAL PENTOBARBITAL ACTION POTENTIALS ELECTRIC STIMULATION RATS, WISTAR