Author/Editor		Gupta, Ramesh C; Milatović, Dejan; Živin, Marko; Dettbarn, Wolf D
Title		Seizure-induced changes in energy metabolites and effects of N-tert-butyl-alpha-phenylnitrone (PNB) and vitamin E in rats
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 440, št. 5 Suppl
Publication year		2000
Volume		str. R160-2
Language		eng
Abstract		Impaired energy metabolism may play a critical role in the neuronal injury caused by kainic acid (KA) induced status epilepticus (SE). Following an acute dose of KA (15 mg/kg, sc) rats developed SE within 1 h. Rats were sacrificed 1 or 72 h after the onset of SE using a head focused microwave technique and the brain regions (pyriform cortex, amygdala, and hippocampus) were assayed for energy metabolites: ATP, ADP, AMP, phosphocreatine (PCr) and creatine (Cr) using reversed-phase HPLC (RP-HPLC). Control values were significantly higher in cortex (23-32%) than in other brain regions. Within 1 h, SE caused a marked decline in ATP (44-56%), PCr (49-64%), total adenine nucleotides (TAN, 45-50%) and total creative compounds (TCC, 32-51%). Within three days, the hippocampus showed the greatest recovery, as the reduced values returned to normal. Pretreatment of rats with an antioxidant (PBN, 200 mg/kg, ip, 30 min prior to KA; or vitamin E (Vit-E), 100 mg/kg, ip/day for 3 days), which did not prevent seizure activity, attenuated depletion of high-energy phosphates caused by KA. These findings suggest that the depletion of energy metabolites caused by KA induced seizures may be linked to oxidative stress mediated toxicity.
Descriptors		ENERGY METABOLISM STATUS EPILEPTICUS KAINIC ACID VITAMIN E BRAIN CHEMISTRY RATS, SPRAGUE-DAWLEY CREATININE ADENOSINE TRIPHOSPHATE ADENOSINE DIPHOSPHATE